CYFIP1/2 Polyclonal antibody

CYFIP1/2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (2)

Cat no : 16011-1-AP

Synonyms

CYFIP2, PIR121, p53-inducible protein 121, p53 inducible protein 121, KIAA1168

---

Validation Data Gallery

• 

  WB analysis of human brain using 16011-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16011-1-AP

  IP result of anti-CYFIP1/2 (IP:16011-1-AP, 4ug; Detection:16011-1-AP 1:700) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 16011-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CYFIP1/2 (16011-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16011-1-AP targets CYFIP1/2 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYFIP1/2 fusion protein Ag8749
• Full Name: cytoplasmic FMR1 interacting protein 2
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC011762
• Gene Symbol: CYFIP2
• Gene ID (NCBI): 26999
• RRID: AB_2090638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

Publications

Species	Application	Title
human	WB	Dis Markers; Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.; Authors - Nachmany Henny H
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Bourcier (Verified Customer) (09-23-2024)

good antibodies

• Applications: Immunoprecipitation
• Cell Tissue Type: Fibroblastes

FH

Lisa (Verified Customer) (03-17-2022)

The antibody works well for WB. Dilution 1:1000 in TBS-T 5% BSA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuronal cells