Anticorps Polyclonal de lapin anti-NANOG

• Phare
• Validé par KD/KO

NANOG Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

224

Avis (3)

N° de cat : 14295-1-AP

Synonymes

hNanog

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Galerie de données de validation

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  WB analysis using 14295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14295-1-AP (NANOG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human embronic stem cells using 14295-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 14295-1-AP at dilution of 1:200. The PE shows staining with 14295-1-AP/PE. The DAPI shows nuclear staining by DAPI.

• 

  FC experiment of NICCT using 14295-1-AP

  1X10^6 NCCIT cells were intracellularly stained with 0.4 ug Anti-Human NANOG (14295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules NCCIT, tissu cérébral de rat, tissu cérébral de souris, tissu embryonnaire de souris
• Résultats positifs en IF/ICC: cellules souches embryonnaires humaines
• Résultats positifs en FC (Intra): cellules NCCIT

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 160 publications below
• IHC: See 19 publications below
• IF: See 69 publications below

Informations sur le produit

14295-1-AP cible NANOG dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NANOG Protéine recombinante Ag5645
• Nom complet: Nanog homeobox
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC160187
• Symbole du gène: NANOG
• Identification du gène (NCBI): 79923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publications

Species	Application	Title
		Cell Stem Cell; A combined cell and gene therapy approach for homotopic reconstruction of midbrain dopamine pathways using human pluripotent stem cells.; Authors - Niamh Moriarty
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
mouse	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
mouse		Mol Cell; RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.; Authors - Xianju Bi
mouse	IF	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: mESCs

NANOG Antibody Western Blot validation (1-1000 dilution) in mESCs (Cat no:14295-1-AP)

FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

NANOG Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:14295-1-AP)

FH

Ying (Verified Customer) (08-19-2019)

very good antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse primary cardiomyocytes

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