Anticorps Polyclonal de lapin anti-MYH7-specific

• Phare
• Validé par KD/KO

MYH7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

57

N° de cat : 22280-1-AP

Synonymes

Myosin 7, MYH7, Myosin heavy chain 7, MyHC-slow, MyHC-beta

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Galerie de données de validation

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  WB analysis of mouse heart using 22280-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22280-1-AP (MYH7-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22280-1-AP

  IP result of anti-MYH7-specific (IP:22280-1-AP, 5ug; Detection:22280-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse heart using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 22280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using MYH7-specific antibody (22280-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF/ICC: cellules H9C2,

Dilution recommandée

• Western Blot (WB): WB : 1:600-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 49 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Informations sur le produit

22280-1-AP cible MYH7-specific dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 7, cardiac muscle, beta
• Masse moléculaire calculée: 223 kDa
• Poids moléculaire observé: 200-220 kDa
• Numéro d’acquisition GenBank: NM_000257
• Symbole du gène: MYH7
• Identification du gène (NCBI): 4625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs. (2523412, 14520662)

Publications

Species	Application	Title
human	WB	Science; HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.; Authors - John T Hinson
mouse	WB	Circulation; Hyperglycemia-Driven Inhibition of AMP-Activated Protein Kinase α2 Induces Diabetic Cardiomyopathy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes In Vivo.; Authors - Shengnan Wu
		Dev Cell; Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.; Authors - Anant Chopra
human	WB	Elife; Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp; Authors - Abigail C Neininger-Castro ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Dectin-1 deficiency alleviates diabetic cardiomyopathy by attenuating macrophage-mediated inflammatory response; Authors - Na Yang
pig	WB	J Agric Food Chem; Dietary Taurine Supplementation Improves the Meat Quality, Muscle Fiber Type, and Mitochondrial Function of Finishing Pigs; Authors - Wenting An

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