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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-AEG-1/MTDH-Specific

AEG-1/MTDH-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

N° de cat : 13860-1-AP

Synonymes

3D3, 3D3/LYRIC, AEG 1, AEG1, AEG-1/MTDH-Specific, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC



Applications testées

Résultats positifs en WBcellules MCF-7, cellules A375, cellules A549, cellules DU 145, cellules HeLa, cellules HepG2, cellules HL-60, cellules PC-3, cellules RAW 264.7, tissu splénique de souris
Résultats positifs en IPcellules MCF-7
Résultats positifs en IHCtissu de cancer du sein humain, tissu cérébral de souris, tissu de cancer du foie humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules MCF-7,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13860-1-AP cible AEG-1/MTDH-Specific dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène AEG-1/MTDH-Specific Protéine recombinante Ag4840
Nom complet metadherin
Masse moléculaire calculée 582 aa, 64 kDa
Poids moléculaire observé 65-70 kDa
Numéro d’acquisition GenBankBC045642
Symbole du gène AEG1/MTDH
Identification du gène (NCBI) 92140
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Protocole

Product Specific Protocols
WB protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IHC protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IF protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
IP protocol for AEG-1/MTDH-Specific antibody 13860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB, IP, IHC

J Crohns Colitis

MTDH promotes intestinal inflammation by positively regulating TLR signaling.

Authors - Lijuan Wang
humanWB

Oncogene

MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.

Authors - He X-X XX

Mol Ther

Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells.

Authors - Hao Teng
humanWB

Mol Ther Nucleic Acids

PMID: 28624193

Authors - Yin-Ping Fan
humanWB,IHC

Oncotarget

MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.

Authors - Jing-Jun Yan
humanIHC

Int J Cancer

Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma.

Authors - Liu Yong Y

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

eman (Verified Customer) (11-26-2020)

this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000 and 1:2000
  • Cell Tissue Type: Hela cells
AEG-1/MTDH-Specific Antibody Western Blot,Immunoprecipitation validation (1:1000 and 1:2000 dilution) in Hela cells (Cat no:13860-1-AP)