BCCIP Polyclonal antibody

• Featured Product
• KD/KO Validated

BCCIP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16043-1-AP

Synonyms

TOK1, TOK 1, Protein TOK-1, Protein TOK 1, P21- and CDK-associated protein 1

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Validation Data Gallery

• 

  WB analysis of HeLa using 16043-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16043-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16043-1-AP

  IP result of anti-BCCIP (IP:16043-1-AP, 4ug; Detection:16043-1-AP 1:800) with HeLa cells lysate 3200ug.

• 

  IF Staining of HeLa using 16043-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16043-1-AP (BCCIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

16043-1-AP targets BCCIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCCIP fusion protein Ag8934
• Full Name: BRCA2 and CDKN1A interacting protein
• Calculated Molecular Weight: 314 aa, 36 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC009771
• Gene Symbol: BCCIP
• Gene ID (NCBI): 56647
• RRID: AB_2063712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; The Males Absent on the First (MOF) Mediated Acetylation Alters the Protein Stability and Transcriptional Activity of YY1 in HCT116 Cells; Authors - Tingting Wu
human	WB	Int J Mol Sci; Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells; Authors - Junaid Ali Shah
human	WB,IF	FEBS J; BCCIP binds to and activates its promoter in a YY1-dependent fashion in HCT116 cells.; Authors - Yi Sui ; KD Validated
human	WB,IF	Protein Cell; Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells.; Authors - Jiaming Su
human	WB	Biochem Biophys Res Commun; BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma.; Authors - Fang Wang
human	WB,IHC	Int J Oncol; Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues.; Authors - Liu Xiaoxia X