Anticorps Polyclonal de lapin anti-MCC

• Phare
• Validé par KD/KO

MCC Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11525-1-AP

Synonymes

MCC, MCC1, mutated in colorectal cancers, Protein MCC

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Galerie de données de validation

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  WB analysis of fetal human brain using 11525-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11525-1-AP

  WB result of MCC antibody (11525-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCC transfected HeLa cells.

• 

  WB analysis of human brain using 11525-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11525-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11525-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11525-1-AP

  IP result of anti-MCC (IP:11525-1-AP, 3ug; Detection:11525-1-AP 1:500) with PC-3 cells lysate 5000ug.

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  IHC staining of human prostate cancer using 11525-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11525-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 11525-1-AP

  Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules HeLa, cellules L02, tissu cérébral humain, tissu hépatique de souris
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

11525-1-AP cible MCC dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCC Protéine recombinante Ag2089
• Nom complet: mutated in colorectal cancers
• Masse moléculaire calculée: 829 aa, 93 kDa
• Poids moléculaire observé: 98 kDa, 113-118 kDa
• Numéro d’acquisition GenBank: BC018919
• Symbole du gène: MCC
• Identification du gène (NCBI): 4163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,

Publications

Species	Application	Title
human	IF	Oncogene; 'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.; Authors - F A Benthani
human	WB	Proteomics; Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.; Authors - Corbo Claudia C
human,mouse	WB,IHC,IF	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song ; KD Validated
mouse	IHC	PLoS One.; Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.; Authors - Rohrbeck A
human	WB	bioRxiv; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan

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