Anticorps Polyclonal de lapin anti-MAD2L2

• Phare
• Validé par KD/KO

MAD2L2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 12683-1-AP

Synonymes

hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog

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Galerie de données de validation

• 

  WB analysis of human brain using 12683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12683-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12683-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12683-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12683-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12683-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12683-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12683-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12683-1-AP

  IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human ovary tumor using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules A375, cellules HEK-293 fœtales, cellules HeLa, cellules K-562, tissu cérébral de souris, tissu de côlon humain, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12683-1-AP cible MAD2L2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAD2L2 Protéine recombinante Ag3373
• Nom complet: MAD2 mitotic arrest deficient-like 2 (yeast)
• Masse moléculaire calculée: 211 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC015244
• Symbole du gène: MAD2L2
• Identification du gène (NCBI): 10459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Publications

Species	Application	Title
human	WB	Nat Commun; MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.; Authors - Inge de Krijger ; KO Validated
mouse	WB	Nat Commun; SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.; Authors - Estelle Vincendeau
human	WB, IP	J Cell Biol; Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.; Authors - Tamar Listovsky ; KD Validated
human	WB	Nucleic Acids Res; REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ; Authors - Junya Tomida ; KD Validated
human	WB	Nucleic Acids Res; REV1 and polymerase ζ facilitate homologous recombination repair.; Authors - Sharma Shilpy S ; KD Validated
human	WB	Nucleic Acids Res; Regulation of error-prone translesion synthesis by Spartan/C1orf124.; Authors - Kim Myoung Shin MS

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