PRCC Polyclonal antibody

PRCC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12019-1-AP

Synonyms

PRCC, Proline rich protein PRCC, RCCP1, TPRC

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Validation Data Gallery

• 

  WB analysis of HeLa using 12019-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12019-1-AP (PRCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12019-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12019-1-AP (PRCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12019-1-AP targets PRCC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRCC fusion protein Ag2638
• Full Name: papillary renal cell carcinoma (translocation-associated)
• Calculated Molecular Weight: 491 aa, 52 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC010450
• Gene Symbol: PRCC
• Gene ID (NCBI): 5546
• RRID: AB_2169667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRCC, also named as, TPRC,regulate cell cycle progression through interaction with MAD2L2. It is frequently found in papillary renal cell carcinoma. Endogenous PRCC is coimmunoprecipitated by antibodies that recognize a variety of pre-mRNA plicing factors including SC35, PRL1 and CDC5.