YY1AP1 Polyclonal antibody

YY1AP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10425-1-AP

Synonyms

YY1AP, YY1 associated protein 1, YAP, Hepatocellular carcinoma-associated protein 2, HCCA2

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Validation Data Gallery

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  WB analysis of MDA-MB-231 using 10425-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 10425-1-AP (YY1AP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 10425-1-AP

  Immunofluorescent analysis of HepG2 cells, using YY1AP1 antibody 10425-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

10425-1-AP targets YY1AP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YY1AP1 fusion protein Ag0692
• Full Name: YY1 associated protein 1
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 75 kDa and 50 kDa
• GenBank Accession Number: BC003500
• Gene Symbol: YY1AP1
• Gene ID (NCBI): 55249
• RRID: AB_2218515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY1AP1 (YY1 associated protein 1), also known as HCCA2 (hepatocellular carcinoma-associated protein 2) or YY1AP, is a 796 amino acid protein that localizes to both the nucleus and the cytoplasm and exists as seven alternatively spliced isoforms. We detected 75 kDa and 50 kDa two isoforms. YY1AP1 is expressed ubiquitously with notable expression in lung, brain, spleen, pancreas, small intestine, heart, colon and skeletal muscle, YY1AP1 interacts with and enhances the transcriptional activation activity of YY1. YY1AP1 is expressed at high levels in liver carcinomas, suggesting a role in tumor transformation and metastasis. YY1AP1 may play a role in cell cycle regulation.

Publications

Species	Application	Title
human	WB,IF	Cell Death Differ; Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.; Authors - Jing Huang
mouse	WB,IF	Psychol Aging; Cognitive engagement and cognitive aging: is openness protective; Authors - Emily Schoenhofen Sharp
mouse	WB	Eur J Pharmacol; Cannabidiol represses miR-143 to promote cardiomyocyte proliferation and heart regeneration after myocardial infarction; Authors - Zhongyu Ren