Anticorps Polyclonal de lapin anti-L-VOCC

L-VOCC Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 21774-1-AP

Synonymes

CACNA1C, CCHL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CACNL1A1, CACN2

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Galerie de données de validation

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  IHC staining of mouse brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 21774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 21774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 21774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21774-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human L-VOCC (21774-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

21774-1-AP cible L-VOCC dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: L-VOCC Protéine recombinante Ag16455
• Nom complet: calcium channel, voltage-dependent, L type, alpha 1C subunit
• Masse moléculaire calculée: 2135 aa, 239 kDa
• Numéro d’acquisition GenBank: BC146846
• Symbole du gène: CACNA1C
• Identification du gène (NCBI): 775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Phytomedicine; Protective role and mechanism of snakegourd peel against myocardial infarction in rats.; Authors - Guanlin Yang
mouse	WB	Antioxid Redox Signal; Anthracycline-induced atrial structural and electrical remodeling characterizes early cardiotoxicity and contributes to atrial conductive instability and dysfunction.; Authors - Ruopeng Tan
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang
rat	WB	Mol Nutr Food Res; Quercetin Attenuates Ethanol-Induced Iron Uptake and Myocardial Injury by Regulating the Angiotensin II-L-Type Calcium Channel.; Authors - Xiaoping Guo
human,mouse	WB	Commun Biol; Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling.; Authors - Yaxiong Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: glandular lumen

L-VOCC Antibody Immunohistochemistry validation (1:100 dilution) in glandular lumen (Cat no:21774-1-AP)

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