CACNA1B Polyclonal antibody

CACNA1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19681-1-AP

Synonyms

BIII, Brain calcium channel III, CACH5, CACNA1B, CACNL1A5, CACNN, Cav2.2

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Validation Data Gallery

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  IHC staining of mouse brain using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19681-1-AP (CACNA1B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19681-1-AP (CACNA1B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19681-1-AP (CACNA1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19681-1-AP (CACNA1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

19681-1-AP targets CACNA1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
• Calculated Molecular Weight: 262 kDa
• GenBank Accession Number: NM_000718
• Gene Symbol: CACNA1B
• Gene ID (NCBI): 774
• RRID: AB_10638918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACNA1B, also named as CACH5, CACNL1A5 and BIII, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). CACNA1B may play a role in directed migration of immature neurons.

Publications

Species	Application	Title
rat	WB	Mol Neurobiol; Genomic Action of Sigma-1 Receptor Chaperone Relates to Neuropathic Pain.; Authors - Shao-Ming Wang
human	WB	Front Pharmacol; Sigma-1 Receptor Plays a Negative Modulation on N-type Calcium Channel.; Authors - Kang Zhang
human	WB	J Recept Signal Transduct Res; CACNA1B facilitates breast cancer cell growth and migration by regulating cyclin D1 and EMT: the implication of CACNA1B in breast cancer.; Authors - Yong-Mei Jin
human	WB	Cell Death Dis; Cav2.2-NFAT2-USP43 axis promotes invadopodia formation and breast cancer metastasis through cortactin stabilization; Authors - Ying Xue ; KO Validated

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