CACNA1D Polyclonal antibody

CACNA1D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 22276-1-AP

Synonyms

CACH3, CACN4, CACNA1D, CACNL1A2, Cav1.3, CCHL1A2

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Validation Data Gallery

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  WB analysis of HEK-293 using 22276-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22276-1-AP (CACNA1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 22276-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22276-1-AP (CACNA1D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 22276-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22276-1-AP (CACNA1D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 22276-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 22276-1-AP (CACNA1D antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22276-1-AP targets CACNA1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium channel, voltage-dependent, L type, alpha 1D subunit
• Calculated Molecular Weight: 248 kDa
• Observed Molecular Weight: 200-250 kDa
• GenBank Accession Number: NM_000720
• Gene Symbol: CACNA1D
• Gene ID (NCBI): 776
• RRID: AB_2879057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (10-12-2022)

AB works well in rat aortic tissue. 1:500 dilution over night.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat aortic tissue