Anticorps Polyclonal de lapin anti-KLF4

• Phare
• Validé par KD/KO

KLF4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA, IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

123

N° de cat : 11880-1-AP

Synonymes

Krueppel-like factor 4, Krueppel like factor 4, GKLF, EZF

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Galerie de données de validation

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  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 11880-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11880-1-AP

  IP result of anti-KLF4 (IP:11880-1-AP, 4ug; Detection:11880-1-AP 1:8000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of mouse embryo using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse embryo using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human embronic stem cells using 11880-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11880-1-AP at dilution of 1:50. The PE shows staining with 11880-1-AP/PE. The DAPI shows nuclear staining by DAPI.

• 

  FC experiment of HeLa using 11880-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KLF4 (11880-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules C2C12, cellules HEK-293, cellules HeLa, cellules HT-1080, cellules HT-29, cellules HUVEC, cellules NCCIT, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu embryonnaire de souris, tissu amygdalien humain, tissu de côlon humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules souches embryonnaires humaines
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 17 publications below
• WB: See 97 publications below
• IHC: See 18 publications below
• IF: See 23 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• ChIP: See 11 publications below

Informations sur le produit

11880-1-AP cible KLF4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLF4 Protéine recombinante Ag2480
• Nom complet: Kruppel-like factor 4 (gut)
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC030811
• Symbole du gène: KLF4
• Identification du gène (NCBI): 9314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4 (PMID: 15561714), with MW range from 50 to 60 kDa,and major band is 55 kDa. KLF4 protein can be ubiquitin modified.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye
human	WB,IHC	Nat Commun; LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.; Authors - Pingping Zhu
human	WB,IHC	J Hematol Oncol; A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.; Authors - Shuiqing Liu
human	IF	Mater Today Bio; Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles.; Authors - Mei-Hwa Lee
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
mouse	IF,WB	J Neuroinflammation; The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke; Authors - Cong Wang

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