- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-JAK2
JAK2 Polyclonal Antibody for WB, IP, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (3)
Applications
WB, IP, IF, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 17670-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules Jurkat, cellules NIH/3T3 |
| Résultats positifs en IP | cellules Jurkat |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 3 publications below |
| WB | See 80 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Informations sur le produit
17670-1-AP cible JAK2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | rat, Humain, poisson-zèbre, porc, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | JAK2 Protéine recombinante Ag11264 |
| Nom complet | Janus kinase 2 (a protein tyrosine kinase) |
| Masse moléculaire calculée | 1132 aa, 130 kDa |
| Poids moléculaire observé | 150 kDa |
| Numéro d’acquisition GenBank | BC039695 |
| Symbole du gène | JAK2 |
| Identification du gène (NCBI) | 3717 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for JAK2 antibody 17670-1-AP | Download protocol |
| IHC protocol for JAK2 antibody 17670-1-AP | Download protocol |
| IP protocol for JAK2 antibody 17670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neuroinflammation Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders | ||
Cell Death Dis 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer | ||
Theranostics Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief. | ||
Arch Med Res Extracellular Vesicles from Carcinoma-associated Fibroblasts Promote EMT of Salivary Adenoid Cystic Carcinoma Via IL-6 | ||
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. | ||
Brain Behav Immun Interleukin-6: Its role and mechanisms in rescuing depression-like behaviors in rat models of depression. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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