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  • KD/KO Validated

STAT5A/B Polyclonal antibody

STAT5A/B Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 13179-1-AP



Tested Applications

Positive WB detected inDaudi cells, K-562 cells, DU 145 cells, 3T3-L1 cells, SGC-7901 cells
Positive IP detected in3T3-L1 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13179-1-AP targets STAT5A/B in WB,IP,IHC,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, cow
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAT5A/B fusion protein Ag3846
Full Name signal transducer and activator of transcription 5A
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight 90-95 kDa, 50-55 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against 445-794 aa of human STAT5A, the homology between this segment and the corresponding segment of human STAT5B is 92%. It shows that this antibody can cross-react with STAT5B.


Product Specific Protocols
WB protocol for STAT5A/B antibody 13179-1-APDownload protocol
IHC protocol for STAT5A/B antibody 13179-1-APDownload protocol
IP protocol for STAT5A/B antibody 13179-1-APDownload protocol
FC protocol for STAT5A/B antibody 13179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.

Authors - Yuezhu Zhang
  • KD Validated


A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration

Authors - Ruonan Li

Front Oncol

In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer

Authors - Xiaodi Gong

Ecotoxicol Environ Saf

Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.

Authors - Yuezhu Zhang

J Cell Mol Med

IL-27 Rα+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation.

Authors - Shanshan Zhao

Cancer Cell Int

LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway.

Authors - Jianxin Lv