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SOCS3 Polyclonal antibody

SOCS3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14025-1-AP

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14025-1-AP targets SOCS3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOCS3 fusion protein Ag5170
Full Name suppressor of cytokine signaling 3
Calculated molecular weight 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC060858
Gene symbol SOCS3
Gene ID (NCBI) 9021
RRIDAB_10597854
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.

Protocols

Product Specific Protocols
WB protocol for SOCS3 antibody 14025-1-APDownload protocol
IP protocol for SOCS3 antibody 14025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Redox Biol

TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes

Authors - Marwa Ben-Khemis
mouseWB

Cell Death Differ

TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation.

Authors - Chang C-P CP
mouseWB,IHC

Kidney Int

Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.

Authors - Nathan Susnik
mouseWB

Oncoimmunology

Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment.

Authors - Liang Fang
humanWB

PLoS Pathog

Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication

Authors - Yi-Sheng Kao
mouseWB

PLoS Genet

PMID: 27711113

Authors - Xiaolin Lin