Anticorps Polyclonal de lapin anti-Integrin beta-6-Specific

Integrin beta-6-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 19695-1-AP

Synonymes

Integrin beta 6, Integrin beta-6-Specific, integrin, beta 6, ITGB6

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Galerie de données de validation

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  WB analysis of A549 using 19695-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 19695-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 19695-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 19695-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 19695-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19695-1-AP (ITGB6-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 19695-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 19695-1-AP (Integrin beta-6-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A2780
• Résultats positifs en IHC: tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

19695-1-AP cible Integrin beta-6-Specific dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: integrin, beta 6
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: NM_000888
• Symbole du gène: Integrin beta 6
• Identification du gène (NCBI): 3694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. The antibody is specific to ITGB6.

Publications

Species	Application	Title
rat,mouse	WB	Glia; αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo.; Authors - Kathleen K Catignas
human	IHC	Cell Biosci; Protein expression of eIF4E and integrin αvβ6 in colon cancer can predict clinical significance, reveal their correlation and imply possible mechanism of interaction.; Authors - Zhengchuan Niu
human	WB,IF,IP	J Exp Clin Cancer Res; Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.; Authors - Feikai Lin
mouse	IHC,IF	Mol Cell Endocrinol; Integrin β6 mediates epithelial-mesenchymal transition in diabetic kidney disease; Authors - Xiaoyu Li
human	WB,IHC	Adv Clin Exp Med; Upregulation of ITGB6 in primary palmar hyperhidrosis; Authors - Jian-Bo Lin
human	WB	Nat Commun; A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability; Authors - Ke Li

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