- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-INO80
INO80 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IF, IHC, ChIP, ELISA
Conjugaison
Non conjugué
N° de cat : 18810-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 8 publications below |
| WB | See 15 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
| ChIP | See 4 publications below |
Informations sur le produit
18810-1-AP cible INO80 dans les applications de WB, IP, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | INO80 homolog (S. cerevisiae) |
| Masse moléculaire calculée | 1556 aa, 177 kDa |
| Poids moléculaire observé | 178 kDa |
| Numéro d’acquisition GenBank | BC146785 |
| Symbole du gène | INO80 |
| Identification du gène (NCBI) | 54617 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for INO80 antibody 18810-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet Mammalian SWI/SNF continuously restores local accessibility to chromatin.
| ||
Cell Stem Cell INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.
| ||
Mol Cell Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin. | ||
Mol Cell Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
| ||
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Nat Commun Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH G (Verified Customer) (06-13-2022) | INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.
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