UCHL5 Polyclonal antibody

• Featured Product
• KD/KO Validated

UCHL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11527-1-AP

Synonyms

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5

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Validation Data Gallery

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  WB analysis of mouse colon using 11527-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11527-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 11527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse brain tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

11527-1-AP targets UCHL5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCHL5 fusion protein Ag2095
• Full Name: ubiquitin carboxyl-terminal hydrolase L5
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC025369
• Gene Symbol: UCHL5
• Gene ID (NCBI): 51377
• RRID: AB_2877774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).

Publications

Species	Application	Title
human	WB	Front Oncol; Ubiquitin C-Terminal Hydrolase L5 (UCHL5) Accelerates the Growth of Endometrial Cancer via Activating the Wnt/β-Catenin Signaling Pathway.; Authors - Da Liu ; KD Validated
mouse	WB	FASEB J; Caveolin-2 in association with nuclear lamina controls adipocyte hypertrophy; Authors - Moonjeong Choi
human	WB	J Cell Biochem; Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.; Authors - Zainab Sabry Othman Ahmed
human	WB	J Cell Biochem; Proteasome-associated cysteine deubiquitinases are molecular targets of environmental optical brightener compounds.; Authors - Isel Castro
human	IHC	Mol Ther Oncolytics; The UCHL5 inhibitor b-AP15 overcomes cisplatin resistance via suppression of cancer stemness in urothelial carcinoma; Authors - Po-Ming Chow
human	IHC	Am J Cancer Res; Anti-tumor effects of deubiquitinating enzyme inhibitor PR-619 in human chondrosarcoma through reduced cell proliferation and endoplasmic reticulum stress-related apoptosis; Authors - Wei-Chou Lin