YY1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YY1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

37

Cat no : 22156-1-AP

Synonyms

UCRBP, NF-E1, INO80S, INO 80S, Delta transcription factor

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of A549 cells using 22156-1-AP

  A549 cells (shcontrol and shRNA of YY1) were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000.

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  WB analysis of Jurkat using 22156-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22156-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22156-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22156-1-AP

  IP result of anti-YY1 (IP:22156-1-AP, 5ug; Detection:22156-1-AP 1:700) with HeLa cells lysate 880ug.

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  IHC staining of human breast cancer using 22156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (22156-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, A549 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 27 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 11 publications below

Product Information

22156-1-AP targets YY1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YY1 fusion protein Ag17792
• Full Name: YY1 transcription factor
• Calculated Molecular Weight: 414 aa, 45 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC037308
• Gene Symbol: YY1
• Gene ID (NCBI): 7528
• RRID: AB_11182932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.

Publications

Species	Application	Title
human	IF	Sci Transl Med; Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1.; Authors - Pan Gao
human	WB	Mol Cell; Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.; Authors - Wenqi Xu
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang ; KD Validated
human	ChIP	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun
human	WB,IP,ChIP	Free Radic Biol Med; Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.; Authors - Wentao Wang
mouse	IHC	Cancers (Basel); HPV8 Reverses the Transcriptional Output in Lrig1 Positive Cells to Drive Skin Tumorigenesis.; Authors - Adnan Shahzad Syed