Anticorps Polyclonal de lapin anti-Inhibin Beta A

• Phare
• Validé par KD/KO

Inhibin Beta A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10651-1-AP

Synonymes

Activin beta A chain, EDF, FRP, INHBA, Inhibin Beta A, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

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Galerie de données de validation

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  WB analysis using 10651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10651-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10651-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 10651-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10651-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10651-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10651-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 10651-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 10651-1-AP

  Immunofluorescent analysis of A549 cells using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules BGC-823, cellules HepG2, cellules MCF-7, cellules SGC-7901
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 11 publications below
• IF: See 3 publications below

Informations sur le produit

10651-1-AP cible Inhibin Beta A dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Inhibin Beta A Protéine recombinante Ag1001
• Nom complet: inhibin, beta A
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC007858
• Symbole du gène: Inhibin beta A
• Identification du gène (NCBI): 3624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin beta A forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin beta A can also bind the beta B and A isoforms to form activin AB and inhibin, respectively.

Publications

Species	Application	Title
human	IF	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
		Development; Combinatorial actions of Tgfβ and Activin ligands promote oligodendrocyte development and CNS myelination.; Authors - Dipankar J Dutta
mouse	WB	PLoS One; Action mechanism of inhibin α-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice.; Authors - Cai Kailai K
human	IHC,IF	Transl Oncol; Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer; Authors - Nanxin Zheng
		Hepatol Commun; Activin A is a prominent autocrine regulator of hepatocyte growth arrest.; Authors - Srividyameena Haridoss
		BMC Cancer; Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells.; Authors - Holli A Loomans

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