Anticorps Polyclonal de lapin anti-IL-22RA1

IL-22RA1 Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

12

Avis (2)

N° de cat : 13462-1-AP

Synonymes

IL22RA1, IL22R, IL 22RA1, IL 22R alpha 1, IL 22 receptor subunit alpha 1

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Galerie de données de validation

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  WB analysis using 13462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL-22RA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13462-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13462-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IF Staining of human gut using 13462-1-AP

  Staining of IL22RA1 (Cat#13462-1-AP) (2ryAbisA647) on FFPE human gut. The nuclei were counter stained with DAPI (in blue). The IL22RA1 signal is localized in the epithelial compartment which is expected. (Data from Manuel Florez, Laboratory Dre. Naglaa Shoukry, CRCHUM).

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  FC experiment of HepG2 using 13462-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IL-22RA1 (13462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HepG2, cellules HT-29, tissu cardiaque de souris, tissu d'intestin grêle de souris
• Résultats positifs en IF: intestin humain,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

13462-1-AP cible IL-22RA1 dans les applications de WB, IP, IF, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-22RA1 Protéine recombinante Ag4056
• Nom complet: interleukin 22 receptor, alpha 1
• Masse moléculaire calculée: 574 aa, 63 kDa
• Poids moléculaire observé: 63-68 kDa
• Numéro d’acquisition GenBank: BC029273
• Symbole du gène: IL-22RA1
• Identification du gène (NCBI): 58985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-22 (IL-22), a member of the IL-10 family of cytokines, is important for the modulation of tissue responses during inflammation. IL-22 receptor is a heterodimer of the IL-10RB and IL-22RA1. IL-22RA1 belongs to the class II cytokine receptor family. It is expressed in a limited number of tissues including skin, colon, liver, lung, pancreas and kidney. The expression is lack in immune cells such as monocytes, T cells, and NK cells (PMID: 15308104). IL-22RA1 can also form a receptor for IL-20 and IL-24 with IL20RB (PMID: 23793061).

Publications

Species	Application	Title
mouse	FC	PLoS Biol; Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.; Authors - Yu Chen
human	WB	Oxid Med Cell Longev; IL-22 Protects against Biliary Ischemia-Reperfusion Injury after Liver Transplantation via Activating STAT3 and Reducing Apoptosis and Oxidative Stress Levels In Vitro and In Vivo.; Authors - Yi Bai
human	WB	Clin Sci (Lond); HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.; Authors - Jing Li
human	WB	Apoptosis; Elevated IL-22 in psoriasis plays an anti-apoptotic role in keratinocytes through mediating Bcl-xL/Bax.; Authors - Bo Wang
human	IHC	Biochim Biophys Acta Mol Basis Dis; IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C.; Authors - Yang Che
human	WB	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (06-13-2022)

Very good antibody for my western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

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