IL-24 Polyclonal antibody

• Featured Product
• KD/KO Validated

IL-24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26772-1-AP

Synonyms

IL24, Melanoma differentiation-associated gene 7 protein, Interleukin-24, interleukin 24, IL 24

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Validation Data Gallery

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  WB analysis using 26772-1-AP

  Mouse spleen tissues were subjected to SDS PAGE followed by western blot with 26772-1-AP (IL-24 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26772-1-AP (IL-24 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26772-1-AP (IL-24 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 26772-1-AP (IL24 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 26772-1-AP (IL24 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, Mouse spleen tissues
• Positive IHC detected in: human colon tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26772-1-AP targets IL-24 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-24 fusion protein Ag25071
• Full Name: interleukin 24
• Calculated Molecular Weight: 207 aa, 24 kDa
• GenBank Accession Number: BC009681
• Gene Symbol: IL-24
• Gene ID (NCBI): 11009
• RRID: AB_2880630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule.

Publications

Species	Application	Title
mouse	IHC,IF	Elife; A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.; Authors - Jia Li
human	WB	Neoplasia; ZBTB7A Enhances Osteosarcoma Chemoresistance by Transcriptionally Repressing lncRNALINC00473-IL24 Activity.; Authors - Lu Zhang
human	WB	Acta Pharmacol Sin; Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway; Authors - Ji-Xian Xiong ; KD Validated
human	WB	Cell Biol Int; Serum IL-24 combined with CA125 as screening and prognostic biomarkers for NSCLC; Authors - Kai Zhang