Anticorps Monoclonal anti-ST2

ST2 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

7A2A7

N° de cat : 60112-1-Ig

Synonymes

DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1

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Galerie de données de validation

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  WB analysis using 60112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 60112-1-Ig

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60112-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60112-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60112-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60112-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 60112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HEK-293 using 60112-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20ug ST2 antibody (60112-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HSC-T6, cellules PC-12, cellules ROS1728, cellules SGC-7901, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal, tissu de cervelet de rat
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Informations sur le produit

60112-1-Ig cible ST2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ST2 Protéine recombinante Ag2555
• Nom complet: interleukin 1 receptor-like 1
• Masse moléculaire calculée: 63 kDa, 37 kDa, 30 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC030975
• Symbole du gène: IL1RL1
• Identification du gène (NCBI): 9173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Publications

Species	Application	Title
mouse	IF	Immunity; Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis.; Authors - Zuojia Chen
human	WB,IHC	Sci Rep; Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.; Authors - Elena Vianello
human	IF	Sci Rep; Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model.; Authors - Shuhei Kobayashi
mouse	IF	Cancer Biomark; Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model; Authors - Shan Wang
human	IF, WB	Inflamm Res; Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis.; Authors - Jingxi Zhang
pig	IHC	Am J Physiol Renal Physiol; INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS.; Authors - Alejandro R Chade

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