Anticorps Monoclonal anti-IFITM2-specific

• Phare
• Validé par KD/KO

IFITM2-specific Monoclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

22

CloneNo.

3D5F7

Avis (1)

N° de cat : 66137-1-Ig

Synonymes

DSPA2c, IFITM2

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Galerie de données de validation

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  WB analysis of HepG2 using 66137-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of recombinant protein using 66137-1-Ig

  IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (66137-1-Ig) at dilution of 1:10,000.

• 

  WB analysis of HepG2 using 66137-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66137-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 66137-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug IFITM2-specific antibody (66137-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HepG2, Protéine recombinante
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66137-1-Ig cible IFITM2-specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interferon induced transmembrane protein 2 (1-8D)
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: NM_006435
• Symbole du gène: IFITM2
• Identification du gène (NCBI): 10581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.

Publications

Species	Application	Title
human	WB	Cell; Intrinsic Immunity Shapes Viral Resistance of Stem Cells.; Authors - Xianfang Wu ; KO Validated
human	WB	Proc Natl Acad Sci U S A; Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.; Authors - Wan-Lin Wu
human	WB	EMBO J; Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.; Authors - Blandine Monel
human	WB	Proc Natl Acad Sci U S A; mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.; Authors - Guoli Shi
human		Mol Cell Proteomics; HIV-1 Nef changes the proteome of T cells extracellular vesicles depleting IFITMs and other antiviral factors; Authors - Mara E da Silva-Januário
human	WB	PLoS Pathog; p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.; Authors - Xin Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM2 expression in permanent cell lines.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100 (immunofluorescence), 1:1000 (Western blot)
• Cell Tissue Type: Permanent cell lines

Produits associés

IFITM2 Polyclonal antibody

12769-1-AP

IFITM3 Polyclonal antibody

11714-1-AP

IFITM1 Polyclonal antibody

11727-3-AP

IFITM1-Specific Monoclonal antibody

60074-1-Ig

RSAD2 Polyclonal antibody

11833-1-AP

IFITM2/3 Monoclonal antibody

66081-1-Ig