Anticorps Monoclonal anti-ICAM-1

• Phare
• Validé par KD/KO

ICAM-1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

46

CloneNo.

2F9A8

N° de cat : 60299-1-Ig

Synonymes

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

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Galerie de données de validation

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  WB analysis using 60299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60299-1-Ig

  WB result of ICAM1 antibody (60299-1-Ig, 1:50,000) with si-Control and si-ICAM1 transfected Raji cells. .

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  WB analysis of HeLa using 60299-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60299-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 60299-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 60299-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 60299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human lung cancer using 60299-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human lung cancer using 60299-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Daudi using 60299-1-Ig

  1X10^6 Daudi cells were surface stained with 0.4 ug Anti-Human ICAM-1 (60299-1-Ig, Clone:2F9A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules Daudi, cellules HeLa, cellules HepG2, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du poumon humain,
• Résultats positifs en cytométrie: cellules Daudi,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 32 publications below
• IHC: See 12 publications below
• IF: See 10 publications below

Informations sur le produit

60299-1-Ig cible ICAM-1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ICAM-1 Protéine recombinante Ag8309
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC015969
• Symbole du gène: ICAM1
• Identification du gène (NCBI): 3383
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

Publications

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB,IHC	Nat Commun; CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy; Authors - Xue Bai
human	WB, IF	EBioMedicine; Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.; Authors - Peng Zhou
	IF	Redox Biol; Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.; Authors - Zhuqing Li
	WB,IHC	Int J Biol Sci; Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury; Authors - Juan Zhang
human	IF	J Cell Biol; Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1.; Authors - Thomas Bessy

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