ICAM2/CD102 Polyclonal antibody

• Featured Product
• KD/KO Validated

ICAM2/CD102 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10121-2-AP

Synonyms

ICAM2, CD102, ICAM-2, ICAM 2

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Validation Data Gallery

• 

  WB analysis of Jurkat using 10121-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10121-2-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10121-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10121-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, RAW 264.7 cells, mouse brain tissue, HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, mouse spleen tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10121-2-AP targets ICAM2/CD102 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ICAM2/CD102 fusion protein Ag0165
• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 55-80 kDa
• GenBank Accession Number: BC003097
• Gene Symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• RRID: AB_2233415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

Publications

Species	Application	Title
mouse	WB	Front Cell Infect Microbiol; Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion.; Authors - Song-Song Zou
human	WB,IHC	PLoS One; ICAM-2 expression mediates a membrane-actin link, confers a nonmetastatic phenotype and reflects favorable tumor stage or histology in neuroblastoma.; Authors - Yoon Karina Jin KJ ; KD Validated
human	IHC	J Ovarian Res; The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas; Authors - Xinxin Xu
human	IHC	Hum Pathol; Intercellular adhesion molecule 1/2 and E-selectin in plasma cell mastitis: immunohistochemical study of 35 cases.; Authors - Yu Dong
human	IF	Stem Cell Res Ther; MSCs promote the efferocytosis of large peritoneal macrophages to eliminate ferroptotic monocytes/macrophages in the injured endometria; Authors - Jiali Wang