Anticorps Polyclonal de lapin anti-IBA1

• Phare
• Validé par KD/KO

IBA1 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

365

Avis (3)

N° de cat : 10904-1-AP

Synonymes

AIF1, AIF 1, IBA 1, G1, allograft inflammatory factor 1

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Galerie de données de validation

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  IP experiment of THP-1 using 10904-1-AP

  IP result of anti-IBA1 (IP:10904-1-AP, 4ug; Detection:10904-1-AP 1:500) with THP-1 cells lysate 2400 ug.

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  IHC staining of rat brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10904-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IBA1 antibody (10904-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 10904-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (10904-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), MAP2 antibody (17490-1-AP, orange), GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta).

• 

  FC experiment of THP-1 using 10904-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human IBA1 (10904-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral humain, tissu d'amygdalite humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en FC (Intra): cellules THP-1

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• IHC: See 94 publications below
• IF: See 197 publications below

Informations sur le produit

10904-1-AP cible IBA1 dans les applications de IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IBA1 Protéine recombinante Ag1363
• Nom complet: allograft inflammatory factor 1
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: BC009474
• Symbole du gène: IBA1
• Identification du gène (NCBI): 199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of IBA1?

The molecular weight of IBA1 is 16.7 kD.

 What is IBA1?

Ionized calcium-binding adaptor molecule 1 (IBA1), also known as Allograft inflammatory factor-1 (AIF-1), is an inflammation-responsive scaffold protein expressed and secreted from macrophages. Microglia response factor (MRF-1) and daintain are also similar, and likely identical, proteins (PMIDs: 29749461, 9630473, 23792284).

 What is the function of IBA1?

IBA1 is necessary for macrophage survival, and it is also a key molecule in proinflammatory activity (PMID: 29749461).

 Where is IBA1 localized?

IBA1 is a cytoplasmic protein, often expressed in immunocytes, macrophages, and microglia. It can be used as a marker for normal (not 'dark') microglia in brain tissue, as IBA1 is expressed by all microglial cell subpopulations (PMIDs: 29749461, 11943136, 26847266, 9630473).

 Is IBA1 upregulated in active immunophages?

Yes; its expression is associated with inflammatory activity (PMID: 29749461).

 How do IBA1-positive microglia differ from microglia that express less or no IBA1?

'Dark' microglia is a recently described phenotype associated with Alzheimer's disease pathology and chronic stress; these dark microglia express decreased IBA1 (often punctiform), and show distinct ultrastructural differences from 'normal' microglia as well as condensed and electron-dense cytoplasm and nucleoplasm. Normal microglia generally display strong, diffuse expression of IBA1. IBA1-positive microglial processes in normal conditions also have a strong tendency to exclusively contact synaptic elements, such as axon terminals, dendritic spines, and synaptic clefts (PMIDs: 29992181, 26847266).

 How is IBA1 related to Alzheimer's disease and pain?

Exacerbated immunoactivity of IBA1, particularly in proximity to amyloid plaques, is prominently featured in AD pathology. IBA1, along with other microglial markers, are also associated with pain, and robust microglial reactions often follow spinal cord injury (PMIDs: 29992181, 23792284).

Publications

Species	Application	Title
	IHC	Acta Neuropathol; Splicing repression is a major function of TDP-43 in motor neurons.; Authors - Aneesh Donde
		ACS Nano; Nanozyme-Based Bandage with Single-Atom Catalysis for Brain Trauma.; Authors - Ruijuan Yan
mouse	IF	Int J Surg; Bioinformatics analysis identifies CSF1R as an essential gene mediating Neuropathic pain - Experimental research.; Authors - Chao Xu
human	IHC	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
mouse		Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
		Front Behav Neurosci; Neuroinflammation in a Rat Model of Tourette Syndrome.; Authors - Ke Zhongling

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (04-05-2024)

Iba1 (in green) marks clearly my microglia in human brain FFPE cortex.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

IBA1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:10904-1-AP)

FH

Alexandru (Verified Customer) (11-13-2023)

Very pleased with the antibody! I had a claer signal in all my stains.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Mouse ventricle

FH

NX (Verified Customer) (02-27-2023)

A clean antibody to detect endogenous Iba1 from mouse brain lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse brain lysate

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