Anticorps Polyclonal de lapin anti-HSP90

• Phare
• Validé par KD/KO

HSP90 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

286

Avis (4)

N° de cat : 13171-1-AP

Synonymes

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

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Galerie de données de validation

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  WB analysis using 13171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13171-1-AP

  IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with K-562 cells lysate 2000ug.

• 

  IP experiment of HeLa using 13171-1-AP

  IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with HeLa cells lysate 4400ug.

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  IHC staining of mouse testis using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13171-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 13171-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 13171-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug HSP90 Polyclonal antibody (13171-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa, cellules NIH/3T3, cellules PC-12, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules K-562, cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 257 publications below
• IHC: See 12 publications below
• IF: See 16 publications below
• IP: See 14 publications below
• ELISA: See 3 publications below
• CoIP: See 14 publications below
• RIP: See 3 publications below

Informations sur le produit

13171-1-AP cible HSP90 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, poulet, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP90 Protéine recombinante Ag3826
• Nom complet: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Masse moléculaire calculée: 853 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC023006
• Symbole du gène: HSP90
• Identification du gène (NCBI): 3320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Publications

Species	Application	Title
human	WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long
mouse	WB	Cell Metab; IgG is an aging factor that drives adipose tissue fibrosis and metabolic decline; Authors - Lexiang Yu
	CoIP	Nat Cell Biol; LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.; Authors - Gaopeng Li
mouse	WB	Bioact Mater; Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy; Authors - Xin Huang
mouse	WB	Nat Commun; The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells; Authors - Xiaofeng Liao
mouse,rat	WB	Nat Commun; Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury; Authors - Tianbao Ye

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Satyendra (Verified Customer) (07-06-2022)

Works very nicely with CEF, DF-1 and VeroE6 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: VeroE6 cells

FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

HSP90 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:13171-1-AP)

FH

Ekaterini (Verified Customer) (02-16-2022)

The best antibody to use for loading control. Always clear and it is not affected by drug treatments.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ALL cell lines

FH

Xiaoping (Verified Customer) (10-22-2018)

This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human neuron and mouse fibroblast samples

HSP90 Antibody Western Blot, validation (1:1000 dilution) in Human neuron and mouse fibroblast samples (Cat no:13171-1-AP)

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