Anticorps Monoclonal anti-GFP tag
GFP tag Monoclonal Antibody for WB
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Protéine recombinante, Aequorea Victoria
Applications
WB, CoIP
Conjugaison
HRP
CloneNo.
1E10H7
N° de cat : HRP-66002
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | Protéine recombinante |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 8 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
HRP-66002 cible GFP tag dans les applications de WB, CoIP et montre une réactivité avec des échantillons Protéine recombinante, Aequorea Victoria
| Réactivité | Protéine recombinante, Aequorea Victoria |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Protéine recombinante |
| Nom complet | GFP tag |
| Masse moléculaire calculée | 26 kDa |
| Poids moléculaire observé | - |
| Numéro d’acquisition GenBank | U73901 |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | HRP |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Publications
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. | ||
Mol Neurodegener Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease. | ||
Elife Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. | ||
Plant Physiol Upstream Open Reading Frame and Phosphate-Regulated Expression of Rice OsNLA1 Controls Phosphate Transport and Reproduction. | ||
Front Microbiol The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wei (Verified Customer) (09-11-2023) | It works well in our detection of GFP-tagged protein from Arabidopsis
|
FH Aamir (Verified Customer) (01-08-2020) | Worked fine for WB
|
