Anticorps Polyclonal de lapin anti-GAP43

GAP43 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

50

Avis (1)

N° de cat : 16971-1-AP

Synonymes

Neural phosphoprotein B 50, Growth-associated protein 43, growth associated protein 43, B 50, Axonal membrane protein GAP-43

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Galerie de données de validation

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  WB analysis using 16971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16971-1-AP (GAP43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16971-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16971-1-AP (GAP43 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 16971-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16971-1-AP (GAP43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16971-1-AP

  IP result of anti-GAP43 (IP:16971-1-AP, 4ug; Detection:16971-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of mouse brain using 16971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16971-1-AP (GAP43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16971-1-AP (GAP43 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 16971-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GAP43 antibody (16971-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 16971-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GAP43 antibody (16971-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu pulmonaire de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 34 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16971-1-AP cible GAP43 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAP43 Protéine recombinante Ag9294
• Nom complet: growth associated protein 43
• Masse moléculaire calculée: 238 aa, 25 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC007936
• Symbole du gène: GAP43
• Identification du gène (NCBI): 2596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The neuronal growth-associated protein GAP43 is known as neuromodulin, B-50, P-57, F1, and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration and may contribute to presynaptic membrane changes in the adult, leading to the neurotransmitter release, endocytosis and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicted molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.

Publications

Species	Application	Title
rat	WB	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
rat	WB	Mater Today Bio; Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury; Authors - Fangguo Liang
rat	WB	Phytomedicine; Ginsenoside Rb1 protects hippocampal neurons in depressed rats based on mitophagy-regulated astrocytic pyroptosis; Authors - Yannan Li
rat	WB	Environ Pollut; Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats.; Authors - Rundong Liu
rat	WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/500 GAP43. Very strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human primary cortical neurons

GAP43 Antibody Immunohistochemistry validation (1/500 dilution) in Human primary cortical neurons (Cat no:16971-1-AP)

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