- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-FABP4
FABP4 Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (3)
Applications
WB, IF, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 12802-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules RAW 264.7, tissu adipeux de souris, tissu cardiaque de rat, tissu de muscle squelettique de souris |
| Résultats positifs en IHC | tissu de cancer du sein humain, tissu cardiaque humain, tissu cutané de souris, tissu de tumeur ovarienne humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 2 publications below |
| WB | See 82 publications below |
| IHC | See 17 publications below |
| IF | See 11 publications below |
Informations sur le produit
12802-1-AP cible FABP4 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, porc, souris, Hamster, Oie |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | FABP4 Protéine recombinante Ag3912 |
| Nom complet | fatty acid binding protein 4, adipocyte |
| Masse moléculaire calculée | 132 aa, 15 kDa |
| Poids moléculaire observé | 15 kDa |
| Numéro d’acquisition GenBank | BC003672 |
| Symbole du gène | FABP4 |
| Identification du gène (NCBI) | 2167 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for FABP4 antibody 12802-1-AP | Download protocol |
| IHC protocol for FABP4 antibody 12802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate. | ||
Biomaterials MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice. | ||
Cell Rep Uncoupling of Metabolic Health from Longevity through Genetic Alteration of Adipose Tissue Lipid-Binding Proteins. | ||
Stem Cells miR-223 regulates adipogenic and osteogenic differentiation of mesenchymal stem cells through a C/EBPs/miR-223/FGFR2 regulatory feedback loop. | ||
Diabetes Pygo2 Regulates Adiposity and Glucose Homeostasis via β-Catenin-Axin2-GSK3β Signaling Pathway. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marco (Verified Customer) (07-04-2024) | One of the best working antibodies we have
|
FH Huai-Chin (Verified Customer) (05-20-2022) | Western blot on 3T3-L1 cells pre and post adipogenesis. Works pretty well. Strong band in 3T3-L1 cells after adipogenesis.
|
