Anticorps Monoclonal anti-ESR2

• Phare
• Validé par KD/KO

ESR2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

5D2C9

N° de cat : 60197-1-Ig

Synonymes

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Galerie de données de validation

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  WB analysis of MCF-7 using 60197-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 60197-1-Ig

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60197-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules SW-1990, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

60197-1-Ig cible ESR2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ESR2 Protéine recombinante Ag5103
• Nom complet: estrogen receptor 2 (ER beta)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC024181
• Symbole du gène: ESR2
• Identification du gène (NCBI): 2100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).

Publications

Species	Application	Title
human	IHC	J Environ Sci (China); Environmental microcystin exposure triggers the poor prognosis of prostate cancer: Evidence from case-control, animal, and in vitro studies; Authors - Chun Pan
mouse	WB	Environ Pollut; Betulinic acid alleviates zearalenone-induced uterine injury in mice; Authors - Chenglin Yang
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
mouse	WB	Biofactors; Propofol exerts neuroprotective functions by down-regulating microRNA-19a in glutamic acid-induced PC12 cells.; Authors - Shashuang Yu
human	WB	PeerJ; The modulatory properties of Astragalus membranaceus treatment on endometrial cancer: an integrated pharmacological method.; Authors - Qianqian Zhang
human	WB,IF	Mol Biol Rep; Induction of estrogen receptor β-mediated autophagy sensitizes breast cancer cells to TAD1822-7, a novel biphenyl urea taspine derivative.; Authors - Qi Su ; KD Validated

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