Anticorps Polyclonal de lapin anti-ENO1

• Phare
• Validé par KD/KO

ENO1 Polyclonal Antibody for WB, IP, IHC, ELISA, IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, CoIP, ELISA, IF/ICC, FC (Intra)

Conjugaison

Non conjugué

Publications

65

Avis (2)

N° de cat : 11204-1-AP

Synonymes

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

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Galerie de données de validation

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  WB analysis using 11204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11204-1-AP

  WB result of ENO1 antibody (11204-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HeLa cells.

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  WB analysis of mouse skeletal muscle using 11204-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11204-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 11204-1-AP

  IP result of anti-ENO1 (IP:11204-1-AP, 4ug; Detection:11204-1-AP 1:8000) with mouse skeletal muscle tissue lysate 1280 ug.

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  IHC staining of human stomach cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11204-1-AP (ENO1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11204-1-AP (ENO1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11204-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (11204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 11204-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human ENO1 (11204-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, cellules PC-3, cellules SGC-7901, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu hépatique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 54 publications below
• IHC: See 16 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

11204-1-AP cible ENO1 dans les applications de WB, IP, IHC, CoIP, ELISA, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENO1 Protéine recombinante Ag1692
• Nom complet: enolase 1, (alpha)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015641
• Symbole du gène: ENO1
• Identification du gène (NCBI): 2023
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
human	WB,IF,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
mouse	WB,IP	Mol Cell; Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.; Authors - Ina Huppertz
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Nucleic Acids Res; FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.; Authors - Yongwoo Na
mouse	WB	Clin Transl Med; METTL3-mediated N6-methyladenosine exacerbates ferroptosis via m6A-IGF2BP2-dependent mitochondrial metabolic reprogramming in sepsis-induced acute lung injury; Authors - Hao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kihsu (Verified Customer) (06-10-2021)

its gave good looking results in WB

• Applications: Western Blot
• Cell Tissue Type: mouse liver tissues

FH

Kristian (Verified Customer) (06-07-2021)

Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

ENO1 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:11204-1-AP)

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