Anticorps Polyclonal de lapin anti-EMILIN1

EMILIN1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 10643-1-AP

Synonymes

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

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Galerie de données de validation

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  WB analysis of human kidney using 10643-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10643-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10643-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10643-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10643-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10643-1-AP (EMILIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, tissu cérébral humain, tissu placentaire humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10643-1-AP cible EMILIN1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMILIN1 Protéine recombinante Ag1016
• Nom complet: elastin microfibril interfacer 1
• Masse moléculaire calculée: 107 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC007530
• Symbole du gène: EMILIN1
• Identification du gène (NCBI): 11117
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

Publications

Species	Application	Title
human	WB	Cancer Res; Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.; Authors - Genee Y Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

EMILIN1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:10643-1-AP)

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