Anticorps Polyclonal de lapin anti-Desmocollin 2

Desmocollin 2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 13876-1-AP

Synonymes

ARVD11, Cadherin family member 2, CDHF2, desmocollin 2, Desmocollin 3, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3

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Galerie de données de validation

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  WB analysis using 13876-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human oesophagus using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse heart using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu œsophagien humain, tissu cardiaque de souris, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

13876-1-AP cible Desmocollin 2 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Desmocollin 2 Protéine recombinante Ag4956
• Nom complet: desmocollin 2
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC063291
• Symbole du gène: Desmocollin 2
• Identification du gène (NCBI): 1824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelia and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.

Publications

Species	Application	Title
human	IF	Dev Cell; Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.; Authors - Sally Dabelsteen
mouse	WB	Sci Total Environ; Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice.; Authors - Guo-Xiang Zhou
human	IF	Br J Cancer; Mapping of truncated O-glycans in cancers of epithelial and non-epithelial origin.; Authors - Troels Boldt Rømer
human	WB	Bioconjug Chem; Transforms of Cell Surface Glycoproteins Charge Influences Tumor Cell Metastasis via Atypically Inhibiting Epithelial-Mesenchymal Transition Including Matrix Metalloproteinases and Cell Junctions; Authors - Mingzhe Wang
human	IHC	Mol Ther Oncolytics; Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.; Authors - Wenjuan Yang
human	WB,IHC,IF	BMC Cancer; Desmoglein 2 and desmocollin 2 depletions promote malignancy through distinct mechanisms in triple-negative and luminal breast cancer; Authors - Ji-Yuan Han ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jane (Verified Customer) (02-03-2022)

One specific band was found around 120kDa, however, the signal is weak so need to use lower dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Heart tissue

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