Anticorps Polyclonal de lapin anti-CYP51A1

• Phare
• Validé par KD/KO

CYP51A1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(29)

Avis (1)

N° de cat : 13431-1-AP

Synonymes

Cytochrome P450 51A1, Cytochrome P450 14DM, CYPLI, CYPL1, CYP51

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Galerie de données de validation

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  WB analysis using 13431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 13431-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13431-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:1000) with mouse testis tissue lysate 2800ug.

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  IP experiment of mouse heart using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:2000) with mouse heart tissue lysate 2200 ug.

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  IF Staining of PC-3 using 13431-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HuH-7, tissu hépatique de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13431-1-AP cible CYP51A1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP51A1 Protéine recombinante Ag4235
• Nom complet: cytochrome P450, family 51, subfamily A, polypeptide 1
• Masse moléculaire calculée: 509 aa, 57 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC032322
• Symbole du gène: CYP51A1
• Identification du gène (NCBI): 1595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human,mouse	WB	Nat Commun .; MMAB promotes negative feedback control of cholesterol homeostasis; Authors - Leigh Goedeke

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

Clear band with clean background

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

CYP51A1 Antibody Western Blot validation (1:2000 dilution) in Mouse liver (Cat no:13431-1-AP)

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