Anticorps Polyclonal de lapin anti-CYP51A1

• Phare
• Validé par KD/KO

CYP51A1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 13431-1-AP

Synonymes

CP51, CYP51, CYP51A1, CYPL1, CYPLI, Cytochrome P450 14DM, Cytochrome P450 51A1, Cytochrome P45014DM, Cytochrome P450LI, LDM, P450 14DM, P450L1, Sterol 14 alpha demethylase

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Galerie de données de validation

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  WB analysis using 13431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 13431-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13431-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:1000) with mouse testis tissue lysate 2800ug.

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  IP experiment of mouse heart using 13431-1-AP

  IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:2000) with mouse heart tissue lysate 2200 ug.

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  IF Staining of PC-3 using 13431-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HuH-7, tissu hépatique de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 22 publications below

Informations sur le produit

13431-1-AP cible CYP51A1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP51A1 Protéine recombinante Ag4235
• Nom complet: cytochrome P450, family 51, subfamily A, polypeptide 1
• Masse moléculaire calculée: 509 aa, 57 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC032322
• Symbole du gène: CYP51A1
• Identification du gène (NCBI): 1595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human,mouse	WB	Nat Commun .; MMAB promotes negative feedback control of cholesterol homeostasis; Authors - Leigh Goedeke

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

Clear band with clean background

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

CYP51A1 Antibody Western Blot validation (1:2000 dilution) in Mouse liver (Cat no:13431-1-AP)

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