- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-Gamma Cystathionase
Gamma Cystathionase Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (2)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment
Conjugaison
Non conjugué
190
N° de cat : 12217-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris |
Résultats positifs en IP | tissu hépatique de souris, |
Résultats positifs en IHC | tissu de cancer du foie humain, tissu de cancer du sein humain, tissu rénal humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF-P | tissu de cancer du foie humain |
Résultats positifs en IF/ICC | cellules HepG2, |
Résultats positifs en FC (Intra) | cellules MCF-7, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:100-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 20 publications below |
WB | See 166 publications below |
IHC | See 27 publications below |
IF | See 11 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Informations sur le produit
12217-1-AP cible Gamma Cystathionase dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, Lapin, porc, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Gamma Cystathionase Protéine recombinante Ag2872 |
Nom complet | cystathionase (cystathionine gamma-lyase) |
Masse moléculaire calculée | 405 aa, 45 kDa |
Poids moléculaire observé | 40-45 kDa |
Numéro d’acquisition GenBank | BC015807 |
Symbole du gène | Gamma Cystathionase |
Identification du gène (NCBI) | 1491 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.
Protocole
Product Specific Protocols | |
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WB protocol for Gamma Cystathionase antibody 12217-1-AP | Download protocol |
IHC protocol for Gamma Cystathionase antibody 12217-1-AP | Download protocol |
IF protocol for Gamma Cystathionase antibody 12217-1-AP | Download protocol |
IP protocol for Gamma Cystathionase antibody 12217-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation. | ||
Cell Metab Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions. | ||
Cell Metab mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression. | ||
Cell Metab Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. | ||
Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Piruthivi (Verified Customer) (12-09-2019) | Western blot was very clear.
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