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Anticorps Polyclonal de lapin anti-Gamma Cystathionase

Gamma Cystathionase Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment

Conjugaison

Non conjugué

N° de cat : 12217-1-AP

Synonymes

CSE, CGL, EC:4.4.1.2, EC:4.4.1.1, Cystathionine gamma-lyase



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
Résultats positifs en IPtissu hépatique de souris,
Résultats positifs en IHCtissu de cancer du foie humain, tissu de cancer du sein humain, tissu rénal humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu de cancer du foie humain
Résultats positifs en IF/ICCcellules HepG2,
Résultats positifs en FC (Intra)cellules MCF-7,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

12217-1-AP cible Gamma Cystathionase dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, Lapin, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Gamma Cystathionase Protéine recombinante Ag2872
Nom complet cystathionase (cystathionine gamma-lyase)
Masse moléculaire calculée 405 aa, 45 kDa
Poids moléculaire observé 40-45 kDa
Numéro d’acquisition GenBankBC015807
Symbole du gène Gamma Cystathionase
Identification du gène (NCBI) 1491
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.

Protocole

Product Specific Protocols
WB protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
IHC protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
IF protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
IP protocol for Gamma Cystathionase antibody 12217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCell treatment

Cell Metab

Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer

Authors - Hannah N Bell
humanWB

Cell Metab

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Authors - Jiajun Zhu
mouseWB

Cell Metab

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Authors - Saara Forsström
mouseWB

Cell Metab

mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.

Authors - Nahid A Khan
WB

Cell Metab

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Authors - Joni Nikkanen
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Piruthivi (Verified Customer) (12-09-2019)

Western blot was very clear.

  • Applications: Western Blot,