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CHDH Polyclonal antibody
CHDH Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17356-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat liver tissue, mouse kidney tissue, mouse liver tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
17356-1-AP targets CHDH in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CHDH fusion protein Ag10614 |
Full Name | choline dehydrogenase |
Calculated Molecular Weight | 594 aa, 65 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC034502 |
Gene Symbol | CHDH |
Gene ID (NCBI) | 55349 |
RRID | AB_2276253 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.
Protocols
Product Specific Protocols | |
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WB protocol for CHDH antibody 17356-1-AP | Download protocol |
IHC protocol for CHDH antibody 17356-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Betaine is accumulated via transient choline dehydrogenase activation during Mouse oocyte meiotic maturation.
| ||
PLoS One Choline dehydrogenase polymorphism rs12676 is a functional variation and is associated with changes in human sperm cell function. | ||
Transl Oncol Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level. | ||
Sci Adv Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression | ||
Aging (Albany NY) Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wojciech (Verified Customer) (03-23-2023) | A band was detected at around 60 kDa. 30 ug of protein was loaded and probed with the antibody at 1:2000 dilution.
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FH Hua (Verified Customer) (12-04-2018) | 40 ug of mouse liver lysate was loaded onto the gel. The incubation time is over night at 4oC.
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