Anticorps Polyclonal de lapin anti-CHAT

CHAT Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

41

Avis (2)

N° de cat : 20747-1-AP

Synonymes

CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2

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Galerie de données de validation

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  WB analysis using 20747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 20747-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 20747-1-AP

  IP result of anti-CHAT (IP:20747-1-AP, 4ug; Detection:20747-1-AP 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse cerebellum using 20747-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20747-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20747-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20747-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 20747-1-AP (CHAT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 20747-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IF Staining of mouse brain using 20747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu placentaire de souris
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IHC: See 6 publications below
• IF: See 18 publications below
• FC: See 1 publications below

Informations sur le produit

20747-1-AP cible CHAT dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: choline acetyltransferase
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 47-50 kDa, 66-70 kDa
• Numéro d’acquisition GenBank: NM_020549
• Symbole du gène: CHAT
• Identification du gène (NCBI): 1103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively. This antibody can recognize both pCHAT and cCHAT.

Publications

Species	Application	Title
rat	IF	Cell Stem Cell; Deriving Schwann cells from hPSCs enables disease modeling and drug discovery for diabetic peripheral neuropathy; Authors - Homa Majd
human	IF	Nat Chem Biol; Small molecule agonist of mitochondrial fusion repairs mitochondrial dysfunction; Authors - Yingjie Guo
human	IF	PLoS Biol; LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.; Authors - Jongbo Lee
mouse	IF	Stem Cells Transl Med; Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.; Authors - Huipu Yuan
mouse	IF	J Neurosci; Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.; Authors - Antoinette Olivas
human	WB	Int J Mol Sci; Synergistic Effect of the Long-Term Overexpression of Bcl-2 and BDNF Lentiviral in Cell Protecting against Death and Generating TH Positive and CHAT Positive Cells from MSC.; Authors - Paulina Borkowska

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jerome (Verified Customer) (07-20-2022)

we followed this protocol : antigen retrieval buffer: citrate pH 6 (DAKO S1699) wash in TBS 1X Blocking buffer TBS1x +Serum Goat 5%, 1H at RT Primary and secondary antibody diluted in TBS1X Wash primary antibody TBS1X 0.2%tween Secondary antibody GAR 594 ref A11012 Wash secondary antibody TBS1X 0.2%tween Unfortunately, it doesn't work under these conditions.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Mouse spinal cord

FH

Xin (Verified Customer) (01-23-2022)

Good performance in WB (around 70 kd).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

CHAT Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:20747-1-AP)

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