BCHE Polyclonal antibody

BCHE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23854-1-AP

Synonyms

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, butyrylcholinesterase, CHE1, Choline esterase II, Cholinesterase, E1, Pseudocholinesterase

---

Validation Data Gallery

• 

  WB analysis of HeLa using 23854-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23854-1-AP (BCHE Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23854-1-AP (BCHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 23854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23854-1-AP (BCHE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 23854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23854-1-AP (BCHE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 23854-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCHE antibody (23854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

23854-1-AP targets BCHE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCHE fusion protein Ag20888
• Full Name: butyrylcholinesterase
• Calculated Molecular Weight: 602 aa, 68 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC008396
• Gene Symbol: BCHE
• Gene ID (NCBI): 590
• RRID: AB_2879341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCHE(butyrylcholinesterase) is an enzyme expressed in most human tissues. It may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease(PMID:11848688). BCHE is also named as CHE1 and belongs to the type-B carboxylesterase/lipase family. This is a glycoprotein with the molecular weight from 68 kDa to 90 kDa.

Publications

Species	Application	Title
human	WB	Nat Biomed Eng; Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose.; Authors - Yuanyuan Li
goat	WB,IF	Cells; Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase; Authors - Jia-Hao Wang
human	WB,IHC	Front Immunol; Progressions of the correlation between lipid metabolism and immune infiltration characteristics in gastric cancer and identification of BCHE as a potential biomarker; Authors - Shibo Wang