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Anticorps Monoclonal anti-CD7

CD7 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A4E6

N° de cat : 60209-2-Ig

Synonymes

CD7, CD7 molecule, GP40, LEU 9, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, Tp40, TP41

Galerie de données de validation

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  • Western Blot (WB) analysis of Jurkat cells using CD7 Monoclonal antibody (60209-2-Ig)

    WB analysis of Jurkat using 60209-2-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of Jurkat cells using CD7 Monoclonal antibody (60209-2-Ig)

    WB analysis of Jurkat using 60209-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human appendicitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human appendicitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human lymphoma using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human lymphoma using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of mouse thymus using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of mouse thymus using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Applications testées

Résultats positifs en WBcellules Jurkat,
Résultats positifs en IHCtissu d'amygdalite humain, tissu d'appendicite humain, tissu de lymphome humain, tissu de thymus de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochimie (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60209-2-Ig cible CD7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène CD7 Protéine recombinante Ag1805
Nom complet CD7 molecule
Masse moléculaire calculée 240 aa, 25 kDa
Poids moléculaire observé 34 kDa
Numéro d’acquisition GenBankBC009293
Symbole du gène CD7
Identification du gène (NCBI) 924
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocole

Product Specific Protocols
WB protocol for CD7 antibody 60209-2-IgDownload protocol
IHC protocol for CD7 antibody 60209-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols