Anticorps Monoclonal anti-CD47

• Phare
• Validé par KD/KO

CD47 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

CloneNo.

1E1D8

N° de cat : 66304-1-Ig

Synonymes

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

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Galerie de données de validation

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  WB analysis of A549 using 66304-1-Ig

  WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells.

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  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66304-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66304-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66304-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HUVEC using 66304-1-Ig

  1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human CD47 (66304-1-Ig, Clone:1E1D8) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HT-1080, cellules HUVEC, cellules Jurkat, cellules K-562, cellules NCI-H1299, cellules SKOV-3, cellules TF-1, tissu placentaire humain
• Résultats positifs en cytométrie: cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

66304-1-Ig cible CD47 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Ag14132
• Nom complet: CD47 molecule
• Masse moléculaire calculée: 323 aa, 35 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC010016
• Symbole du gène: CD47
• Identification du gène (NCBI): 961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Publications

Species	Application	Title
human,mouse	WB	J Control Release; Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers.; Authors - Mingming Song
mouse	WB	J Nanobiotechnology; Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.; Authors - Hongying Chen
human	WB	RSC Adv; Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage; Authors - Jing Li
human	IF,FC	J Cell Commun Signal; CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles; Authors - Sukhbir Kaur
human	WB	J Cancer Res Clin Oncol; B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy; Authors - Siji Chen
human	WB	J Clin Lab Anal; MicroRNA-148a-3p inhibits cancer progression and is a novel screening biomarker for gastric cancer.; Authors - Chenhui Bao

Produits associés

CD47 Polyclonal antibody

20305-1-AP

Thrombospondin 1 Polyclonal antibody

18304-1-AP

Fibrinogen Gamma Chain Monoclonal antibody

66158-1-Ig

SIRP Alpha Polyclonal antibody

14482-1-AP