SIRP Alpha Polyclonal antibody

SIRP Alpha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14482-1-AP


BIT, CD172A, Inhibitory receptor SHPS 1, Macrophage fusion receptor, MFR, MYD 1, MyD 1 antigen, MYD1, P84, PTPNS1, SHP substrate 1, SHPS 1, SHPS1, SIRP, SIRP alpha, SIRP ALPHA 1, Sirp alpha 2, Sirp alpha 3, SIRPA, SIRPalpha, SIRPalpha2

Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells, PC-12 cells, U-937 cells, mouse cerebellum tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

14482-1-AP targets SIRP Alpha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRP Alpha fusion protein Ag5865
Full Name signal-regulatory protein alpha
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC033092
Gene Symbol SIRP Alpha
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.


Product Specific Protocols
WB protocol for SIRP Alpha antibody 14482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Prolif

Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling

Authors - Qing Tu

Acta Biochim Biophys Sin (Shanghai)

Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6.

Authors - Kejia Zhao

J Obstet Gynaecol Res

Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.

Authors - Qi Xie


Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis

Authors - Ying Jiang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Rebecca (Verified Customer) (05-27-2024)

Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC. Multiple bands observed. A bigger dilution of the antibody is probably needed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: hiPSC-CM
SIRP Alpha Antibody Western Blot validation (1:1000 dilution) in hiPSC-CM (Cat no:14482-1-AP)