Anticorps Polyclonal de lapin anti-CD16

CD16 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 16559-1-AP

Synonymes

CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2

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Galerie de données de validation

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  IHC staining of human liver using 16559-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16559-1-AP (CD16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16559-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16559-1-AP (CD16 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 16559-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16559-1-AP (CD16 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16559-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16559-1-AP (CD16 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 16559-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD16 antibody (16559-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu hépatique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 16 publications below
• FC: See 1 publications below

Informations sur le produit

16559-1-AP cible CD16 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD16 Protéine recombinante Ag9787
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

Publications

Species	Application	Title
human	IF	J Immunother Cancer; Discovery of a glucocorticoid receptor (GR) activity signature correlates with immune cell infiltration in adrenocortical carcinoma; Authors - Kan Wu
human	WB	J Allergy Clin Immunol; Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.; Authors - Yunsheng Liang
rat	IF	Theranostics; Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring.; Authors - Luodan Yang
rat	WB,IF	Theranostics; Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.; Authors - Luodan Yang
human	IF	J Transl Med; Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease; Authors - Beibei Li
mouse	IHC	Front Immunol; Efficacy of MUC1-targeted CAR-NK cells against human tongue squamous cell carcinoma; Authors - Xiaolan Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH9.0.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Prostate

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