Anticorps Polyclonal de lapin anti-CAPRIN1

• Phare
• Validé par KD/KO

CAPRIN1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

68

Avis (3)

N° de cat : 15112-1-AP

Synonymes

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105

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Galerie de données de validation

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15112-1-AP

  IP result of anti-CAPRIN1 (IP:15112-1-AP, 4ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.

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  IHC staining of human breast cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15112-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of OC2 cells using 15112-1-AP

  IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.

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  IF Staining of HeLa using 15112-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 15112-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of cochlear cryosections using 15112-1-AP

  IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with Cochlear cryosections by Dr. Emily Towers. CAPRIN1, purple; DAPI, blue.

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  FC experiment of NIH/3T3 using 15112-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (15112-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2, cellules OC2, cryosections de cochlée
• Résultats positifs en FC (Intra): cellules NIH/3T3
• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 49 publications below
• IHC: See 3 publications below
• IF: See 25 publications below
• IP: See 4 publications below
• RIP: See 2 publications below

Informations sur le produit

15112-1-AP cible CAPRIN1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAPRIN1 Protéine recombinante Ag7099
• Nom complet: cell cycle associated protein 1
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC001731
• Symbole du gène: CAPRIN1
• Identification du gène (NCBI): 4076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
human	WB	Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang ; KD Validated
human	WB,IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
human	WB,IF	Nat Immunol; Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.; Authors - Henan Xu
mouse	WB,RIP	Nat Commun; Fear extinction is regulated by the activity of long noncoding RNAs at the synapse; Authors - Wei-Siang Liau
	WB, IP	Neuron; CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.; Authors - Silvia De Rubeis

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katherine (Verified Customer) (10-31-2023)

The antibody worked well with 1:5000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T

FH

Lisa (Verified Customer) (02-20-2020)

The antibody works very well, giving specific bands in WB and a very good staining in IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: WB:1:2500 - IF:1:100
• Cell Tissue Type: Fibroblasts, lymphoblastoid cell line

CAPRIN1 Antibody Western Blot,Immunofluorescence, validation (WB:1:2500 - IF:1:100 dilution) in Fibroblasts, lymphoblastoid cell line (Cat no:15112-1-AP)

FH

Paul (Verified Customer) (04-30-2019)

Product works very well for WB, IP and IF using rat brain tissue samples.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Rat brain

CAPRIN1 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:2000 dilution) in Rat brain (Cat no:15112-1-AP)

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