Anticorps Polyclonal de lapin anti-C9orf72

• Phare
• Validé par KD/KO

C9orf72 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 25757-1-AP

Synonymes

C9orf72, C9RANT

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Galerie de données de validation

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  WB analysis of HEK-293 using 25757-1-AP

  WB result of C9orf72 antibody (25757-1-AP, 1:250 incubated at RT for 1 hour) with si-C9orf72 HEK293 cells, C9orf72 CRISPR KO HEK293 cells; normal HEK293 cells and non-targeting control (NTC) siRNA transfected HEK293 cells as control. The ~50 kDa is the C9orf72 Long isoform. (Data from Dr Chris Webster, Postdoc in Kurt de Vos’s group at SITRAN).

• 

  WB analysis of mouse brain using 25757-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25757-1-AP (C9orf72 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25757-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25757-1-AP (C9orf72 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human gliomas using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25757-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25757-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25757-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25757-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 25757-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25757-1-AP (C9orf72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de gliome humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1500
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

25757-1-AP cible C9orf72 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C9orf72 Protéine recombinante Ag22723
• Nom complet: chromosome 9 open reading frame 72
• Masse moléculaire calculée: 481 aa, 54 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC068445
• Symbole du gène: C9orf72
• Identification du gène (NCBI): 203228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. This antibody detects the N-terminal of C9orf72.

Publications

Species	Application	Title
human	IHC	Nat Med; Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.; Authors - Yingxiao Shi
human	WB	Acta Neuropathol; An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.; Authors - Claudia S Bauer
human	WB,IF	Elife; Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.; Authors - Carl Laflamme
human,rat	WB	Theranostics; C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α; Authors - Wenzhong Zheng
human	WB	Front Cell Neurosci; Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation; Authors - Claudia S Bauer
human	WB,IF	EMBO Rep; C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains; Authors - Julia Jülg ; KO Validated

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