Anticorps Polyclonal de lapin anti-BTG2

• Phare
• Validé par KD/KO

BTG2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 22339-1-AP

Synonymes

Protein BTG2, PC3, BTG family, member 2

---

Galerie de données de validation

• 

  IHC staining of mouse small intestine using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22339-1-AP (BTG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22339-1-AP (BTG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22339-1-AP (BTG2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22339-1-AP (BTG2 Antibody) at dilution of 1:50.

• 

  IF Staining of Neuro-2a using 22339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using BTG2 antibody (22339-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 22339-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human BTG2 (22339-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Neuro-2a using 22339-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human BTG2 (22339-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,
• Résultats positifs en FC (Intra): cellules SH-SY5Y, cellules Neuro-2a

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

22339-1-AP cible BTG2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BTG2 Protéine recombinante Ag17819
• Nom complet: BTG family, member 2
• Masse moléculaire calculée: 158 aa, 17 kDa
• Numéro d’acquisition GenBank: BC105949
• Symbole du gène: BTG2
• Identification du gène (NCBI): 7832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein BTG2, the anti-proliferative human homolog of murine Pc3/Tis21, belongs to the BTG/Tob family. This family has structurally related proteins that appear to have anti-proliferative properties. It has been demonstrated that BTG2 is p53-inducible and is involved in cell cycle control and cellular response to DNA damage (PMID: 8944033). As a transcriptional co-regulator, BTG2 has been shown to interact with CAF1 and POP2, and works as a co-activator of ERa-mediated transcription via a CCR4-like complex (PMID: 18974182).

Publications

Species	Application	Title
human	WB	Front Oncol; Transcriptomic Analyses Reveal B-Cell Translocation Gene 2 as a Potential Therapeutic Target in Ovarian Cancer.; Authors - Jia Wang ; KD Validated
mouse	WB,IF	FASEB J; MicroRNA-409-3p/BTG2 signaling axis improves impaired angiogenesis and wound healing in obese mice; Authors - Furkan Bestepe
mouse	WB	ACS Chem Biol; Epitranscriptomic N6-Methyladenosine Modification Is Required for Direct Lineage Reprogramming into Neurons.; Authors - Hwan Choi
human	WB	Front Mol Biosci; Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma.; Authors - Aitao Nai
human	WB	J Oncol; lncRNA SNHG5 Modulates Endometrial Cancer Progression via the miR-25-3p/BTG2 Axis.; Authors - Shi Li
human	IHC	Dig Dis Sci; BTG2 Is Down-Regulated and Inhibits Cancer Stem Cell-Like Features of Side Population Cells in Hepatocellular Carcinoma.; Authors - Chen-Song Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, incubated with AF488 at room temperature for 2 h.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovarian tissue

Produits associés

BTG1 Polyclonal antibody

14879-1-AP

PRMT1 Polyclonal antibody

11279-1-AP

P53 Polyclonal antibody

10442-1-AP

BTG4 Polyclonal antibody

20514-1-AP