Anticorps Polyclonal de lapin anti-BIN2

• Phare
• Validé par KD/KO

BIN2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14245-1-AP

Synonymes

BIN2, BRAP 1, BRAP1, bridging integrator 2

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Galerie de données de validation

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  WB analysis of U-937 using 14245-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14245-1-AP

  IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug.

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  IHC staining of human spleen using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules U-937
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu splénique humain, tissu cardiaque de souris, tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14245-1-AP cible BIN2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BIN2 Protéine recombinante Ag5502
• Nom complet: bridging integrator 2
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC047686
• Symbole du gène: BIN2
• Identification du gène (NCBI): 51411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

Publications

Species	Application	Title
mouse	WB,IP	J Clin Invest; BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation.; Authors - Julia Volz ; KO Validated

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