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  • KD/KO Validated

Endophilin 1 Polyclonal antibody

Endophilin 1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12435-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

CNSA2, EEN B1, Endophilin 1, Endophilin A1, SH3 domain GRB2 like 2, SH3 domain protein 2A, SH3D2A, SH3GL2, SH3P4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inC6 cells, mouse brain, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12435-1-AP targets Endophilin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endophilin 1 fusion protein Ag3114 Predict reactive species
Full Name SH3-domain GRB2-like 2
Calculated Molecular Weight 39.8 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC032825
Gene Symbol Endophilin 1
Gene ID (NCBI) 6456
RRIDAB_2187398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Endophilin 1 antibody 12435-1-APDownload protocol
IHC protocol for Endophilin 1 antibody 12435-1-APDownload protocol
IP protocol for Endophilin 1 antibody 12435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior.

Authors - Majumdar Shyama S
  • KD Validated
humanWB

Invest Ophthalmol Vis Sci

Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma

Authors - Shuilian Chen
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